1/ Project Title: Investigating the impacts of squatting on gut microflora and colorectal cancer by 16s rRNA NGS
Supervisor: Dr. Mustak Ibn Ayub
Designation: Assistant Professor
Address: Department of Genetic Engineering and Biotechnology,
University of Dhaka, Dhaka-1000, Bangladesh
E-mail: miayub@du.ac.bd
Phone: +88-01860015272
Timeline: Aug 2021 - July 2022
Project Details click here
Responsibilities
- Sample transport, processing and storage
- Data collection and manage
- Handle study population with all ethical issues
- Design data collection form
- Literature review
- DNA extraction from fecal samples
- Purity analysis with Nanodrop Spectrophotometer
- DNA sample transfer for 16s rRNA Illumina sequencing
- 16s rRNA NGS data analysis
- Maintain regular communication with all collaborators
2/ Project Title (M.Sc Thesis): Expression analysis of KLHDC10, PLEKHD1, ZSWIM5, LRRC3B AP006621.6,
RP11-218M22.1. RP11-467J12.4 and CTD-3032H12.1 genes in Breast cancer patient.
Supervisor: Md. Moniruzzaman
Designation: Scientific Officer
Address: Molecular Biotechnology Division, National Institute of Biotechnology, Dhaka-1349, Bangladesh
E-mail: monirbge06033@gmail.com
Phone: +88-01722223029
Timeline: Jan 2021 - July 2021
Responsibilities
- Sample transport, process and storage
- Literature review
- FFPE sample sectioning with microtome machine
- RNA extraction from breast tumor sample
- Purity analysis with Nanodrop Spectrophotometer
- cDNA synthesis, dilution and purity analysis with Nanodrop
- Real-time quantitative PCR
- Statistical analysis with R programming
- Manuscript preparation
3/ Project Title: Identification and characterization of economically valuable alpine flora of Bangladesh
with Sanger Sequencing.
Supervisor: Keshob Chandra Das
Designation: Principle Scientific Officer & Division Head
Address: Molecular Biotechnology Division, National Institute of Biotechnology
E-mail: keshob@gmail.com
Timeline: Feb 2021 - June 2021
Responsibilities
- Literature review
- DNA extraction from plant samples
- Purity analysis with Nanodrop Spectrophotometer
- Agarose gel electrophoresis
- Gel Visualization
- Gradient PCR and PCR
- PCR Product Purification
- Prepare sampel and run Sanger sequencing with Applied Biosystem Genetic Analyzer
- Bioinformatic analysis
- Project report presention
1/ Executive (Application Support) (Sep 2024 - Present)
I have been working as an Executive for Application Department at Diamed Bangladesh
- Website: http://www.diamedbd.com/
Responsibilities
- Provide technical assistance for running laboratory equipment and onsite sample handling
- Conduct end-user training on human leukocyte antigen (HLA) typing and donor-specific antibody (DSA) tests using the
Luminex xMAP
Technology and submit weekly report to management
- Flow cytometry for cancer diagnosis and monitioring (leukemia, lymphoma, myeloma, MRD)
1/ Research Officer (Aug 2022 - Dec 2023)
I have been working as a Research Officer at Projahnmo
Research Foundation.
- Website: https://prfbd.org
- I am currently working on A Phase 2/3, Randomized, Observer-Blind, Placebo-Controlled
Study to Evaluate the Safety and Efficacy of mRNA-1345, an
mRNA Vaccine Targeting Respiratory Syncytial Virus (RSV), in
Adults ≥ 60 Years of Age
Responsibilities
- Maintain communication with domestic and international delegates
- Prepare and revise research proposals, and SOPs/Protocols
- Weekly study report presentation
- Monitor and supervise all lab activities
- Monitorring sample collection, processing and storage
- Official paperwork, documentation, and manage other activities
- Sample Shipment
- Track and update all data logs on a regular basis
- Maintain logistics inventory and lab equipment calibration
- Follow GLP during all the lab activities
- Guide and train lab technologist to all experimental lab work
2/ Research Assistant (Aug 2021 - July 2022)
Project Title: Investigating the impacts of squatting on gut microflora and colorectal cancer
Supervisor: Dr. Mustak Ibn Ayub
Designation: Assistant Professor
Address: Department of Genetic Engineering and Biotechnology Laboratory,
University of Dhaka, Dhaka-1000, Bangladesh
E-mail: miayub@du.ac.bd
Responsibilities
- Sample collection, processing and storage
- DNA/RNA extraction & quantification
- Agarose gel electrophoresis
- PCR/RT-PCR
- Various media preparation, bacteria culture, and gram staining techniques for microorganism identification
- Data collection and manage
- Preparation of autoclaves, sterile techniques in isolation, and transfer of microorganisms
- Maintain aseptic techniques, hazardous chemical wastes, and laboratory safety protocols
- Handle study population with all ethical issues during data collection
- Literature review
- 16s Illumina NGS data analysis
- Maintain regular communication with all collaborators
3/ Research Associate (Feb 2021 - Jun 2021)
I have worked as a Research Associates on this project:"Identification and characterization of economically
valuable alpine flora of Bangladesh" at Molecular Biotechnology Division of National Institute
of Biotechnology, Savar, Dhaka-1349, Bangladesh.
Responsibilities
- Literature review
- DNA extraction and quantification
- Agarose gel electrophoresis
- PCR
- PCR Product Purification
- Sanger sequencing
- Bioinformatic analysis
- Project report presention
4/ Web Design and Development (June 2017 - Feb 2020)
I have been working as a Professional Web Developer in renowned freelancing marketplace
(
Fiverr.com and
Upwork.com)
My Web Development Services
- WordPress Theme Customization
- Ecommerce website Setup with WooCommerce
- WordPress Website Design From Scratch
- Front-end Development with Bootstrap
- PSD/Figma to HTML/Bootstrap 4 or 5
- WordPress SEO with Yoast SEO/Rank Math/All in one SEO
- Mailchimp
For More Information
Click Here
Research Volunteer Group Leader (Jan 2019 - Feb 2020)
After I have enrolled in Master's program I started working as a research volunteer for my
university's Research Cell Division in Noakhali-3814, Bangladesh, on various research projects.
I led a research team that conducts various types of fieldwork and data collection.
Responsibilities
- Assisting researcher/faculty members in data/sample collection
- Survey, data entry, data analysis, and event (seminar/conference) management.
- I was also responsible to train and supervise junior volunteers on this task
